Expected Data Output

Note:  The sequencing specifications stated below for the Illumina platforms are validated specifications for the control library PhiX. These specifications are not expected for low base diversity libraries, novel libraries or libraries with custom components. Also, one lane/chip is the smallest purchasable unit.

Illumina HiSeq 2500: HighOutput Mode v3

Read Throughput
Single Reads Paired End Reads
Reads/Flowcell 1.5 Billion 3 Billion
Reads/Lane (8 Lanes per Flowcell) 187.5 Million 375 Million

 

Base Throughput
Read Length (bp) Gb (gigabases) per Lane Quality
1×50 9.375 >80% Bases higher than Q30
2x5o or 1×100 18.75  >80% Bases higher than Q30
2×100 37.5  >80% Bases higher than Q30

Illumina HiSeq 2500: Rapid Mode v2

Read Throughput
Single Reads Paired End Reads
Reads/Flowcell 300 Million 600 Million
Reads/Lane (2 Lanes per Flowcell) 150 Million 300 Million

 

Base Throughput
Read Length (bp) Gb (gigabases) per Lane Quality
2×50 12.5-15 >80% Bases higher than Q30
2×100 25-30  >80% Bases higher than Q30
2×150 37.5-45  >75% Bases higher than Q30
2×250  62.5-75 >75% Bases higher than Q30

Illumina NextSeq 500 v2

Read Throughput
HighOutput Mode
Single Reads 400 million
Paired-end Reads 800 million
Mid Output Mode
Single Reads 130 million
Paired-end Reads 260 million

 

HighOutput
Read Length (bp) Total Time Output Quality
1×75 ~ 11 hrs 25-30 Gb >80% Bases higher than Q30
2×75 ~18 hrs 50-60 Gb >80% Bases higher than Q30
2×150 ~29 hrs 100-120 Gb >75% Bases higher than Q30
Mid Output
Read Length (bp) Total Time Output Quality
2×75 ~15 hrs 16-19 Gb >80% Bases higher than Q30
2×150 ~26 hrs 32-39 Gb >75% Bases higher than Q30

Illumina MiSeq

Read Throughput
V2 Reagent Kit Number of Reads
Single Reads 12-15 Million
Paired-end Reads 24-30 Million
V3 Reagent Kit Number of Reads
Single Reads 22-25 Million
Paired-end Reads 44-50 Million

 

Reagent Kit v2
Read Length (bp) Total Time Output Quality
1×36 ~ 4 hrs 540-610 Mb >90% Bases higher than Q30
2×25 ~5.5 hrs 750-850 Mb >90% Bases higher than Q30
2×150 ~24 hrs 4.5-5.1 Gb >80% Bases higher than Q30
2×250 ~ 39 hrs 7.5-8.5 Gb >75% Bases higher than Q30
Reagent Kit v3
Read Length (bp) Total Time Output Quality
2×75 ~24 hrs 3.3-3.8 Gb >85% Bases higher than Q30
2×300 ~65 hrs 13.2-15 Gb >70% Bases higher than Q30

Ion Torrent Personal Genome Machine (PGM)

Read Throughput
Read Length (bp) Ion 314 V2 Chip Ion 316 V2 Chip Ion 318 V2 Chip
200/400 0.4-0.55 Million 2-3 Million 4-5.5 Million

 

Base Throughput
Read Length (bp) Ion 314 V2 Chip Ion 316 V2 Chip Ion 318 V2 Chip
200 30-50 Mb 300-600 Mb  600-1000 Mb
400 60-100 Mb 600-1000 Mb 1200-2000 Mb

Ion Torrent Proton

Read Throughput Read Length (bp) Output Run Time
60-80 Million 200 10GB 4 Hours