Protein ID/Quantification

6-LTQ-Orbitrap-XL-100-weissMass Spec – The department has an LTQ Orbitrap Elite mass spectrometer coupled to a nano-flow Ultra High Pressure liquid chromatography instrument (LC). The LTQ Orbitrap Elite combines a linear ion trap with a Fourier transform mass spectrometer, uses electrospray ionization (liquid samples) and offers a choice between Collision Induced Dissociation and Higher Energy Collision Dissociation. The LC operates in nano-flow mode and is able to work at ultra-high pressure (up to 10,000 psi). Samples can be chromatographically separated in one or two dimensions and subjected to tandem mass spectrometry for protein identification and quantitation. Suitable methods include SILAC, ICAT, iTRAQ and label-free approaches.

 

 

Location: 4th Floor, CGSB