Platforms & Specifications

Platforms

GenCore currently operates three different Illumina sequencing platforms and one Oxford Nanopore Technologies sequencing platform across our two locations.

Specifications

Note: The sequencing specifications stated below are validated specifications for the control library PhiX. These specifications are not expected for low base diversity libraries, novel libraries or libraries with custom components.

NovaSeq 6000

Flow Cell Type
SP
S1
S2
S4
Single Reads
650 - 800 million
1.3 - 1.6 billion
3.3 - 4.1 billion
8 - 10 billion
Paired-end Reads
1.3 - 1.6 billion
2.6 - 3.2 billion
6.6 - 8.2 billion
16 - 20 billion
Base Throughput (Gb)
Read Length (bp)
SP
S1
S2
S4
2 x 50
65 - 80
134 - 167
333 - 417
N/A
2 x 100
N/A
266 - 333
667 - 833
1600 - 2000
2 x 150
200 - 250
400 - 500
1000 - 1250
2400 - 3000
2 x 250
325 - 400
N/A
N/A
N/A

NextSeq 500

Read Throughput
High-Output
Single Reads
400 million
Paired-end Reads
800 million
Mid-Output
Single Reads
130 million
Paired-end Reads
260 million
Base Throughput
High-Output
Read Length (bp)
Total Time
Output (GB)
Quality
1 x 75
11 hours
25 - 30
>80% bases higher than Q30
2 x 75
18 hours
50 - 60
>80% bases higher than Q30
2 x 150
29 hours
100 - 120
>75% bases higher than Q30
Mid-Output
Read Length (bp)
Total Time
Output (GB)
Quality
2 x 75
15 hours
16 - 19
>80% bases higher than Q30
2 x 150
26 hours
32 - 39
>75% bases higher than Q30

MiSeq

Read Throughput
Reagent Kit V3
Single Reads
22 - 25 million
Paired-end Reads
44 - 50 million
Reagent Kit V2
Single Reads
12 - 15 million
Paired-end Reads
24 - 30 million
Base Throughput
Reagent Kit V3
Read Length (bp)
Total Time
Output (GB)
Quality
2 x 75
24 hours
3.3 - 3.8
>85% bases higher than Q30
2 x 300
65 hours
13.2 - 15
>70% bases higher than Q30
Reagent Kit V2
Read Length (bp)
Total Time
Output (GB)
Quality
2 x 150
24 hours
4.5 - 5.1
>80% bases higher than Q30
2 x 250
39 hours
7.5 - 8.5
>75% bases higher than Q30