Platforms

GenCore currently operates four different Illumina sequencing platforms across our two locations.

Specifications

Note: The sequencing specifications stated below are validated specifications for the control library PhiX. These specifications are not expected for low base diversity libraries, novel libraries or libraries with custom components.

NovaSeq 6000

Flow Cell Type
SP
S1
S2
S4
Single Reads
650 - 800 million
1.3 - 1.6 billion
3.3 - 4.1 billion
8 - 10 billion
Paired-end Reads
1.3 - 1.6 billion
2.6 - 3.2 billion
6.6 - 8.2 billion
16 - 20 billion
Base Throughput (Gb)
Read Length (bp)
SP
S1
S2
S4
2 x 50
65 - 80
134 - 167
333 - 417
N/A
2 x 100
N/A
266 - 333
667 - 833
1600 - 2000
2 x 150
200 - 250
400 - 500
1000 - 1250
2400 - 3000
2 x 250
325 - 400
N/A
N/A
N/A

HiSeq 2500 High-Output Mode

Read Throughput
Single Reads
Paired End Reads
Reads per flowcell
1.5 billion
3 billion
Reads per lane (8 lanes per flowcell)
187.5 million
375 million
Read Length (bp)
GB per flowcell
GB per lane
Quality
1 x 50
75
9.375
Greater than 80% of bases above Q30
2 x 50 and 1 x 100
150
18.75
Greater than 80% of bases above Q30
2 x 100
300
37.5
Greater than 80% of bases above Q30

HiSeq 2500 Rapid Run Mode

Read Throughput
Single Reads
Paired End Reads
Reads per flowcell
300 million
600 million
Reads per lane (2 lanes per flowcell)
150 million
300 million
Read Length (bp)
GB per flowcell
GB per lane
Quality
2 x 50
25 - 30
12.5 - 15
Greater than 85% of bases above Q30
2 x 100
50 - 60
25 - 30
Greater than 80% of bases above Q30
2 x 150
75 - 90
37.5 - 45
Greater than 75% of bases above Q30

NextSeq 500

Read Throughput
High-Output
Single Reads
400 million
Paired-end Reads
800 million
Mid-Output
Single Reads
130 million
Paired-end Reads
260 million
Base Throughput
High-Output
Read Length (bp)
Total Time
Output (GB)
Quality
1 x 75
11 hours
25 - 30
>80% bases higher than Q30
2 x 75
18 hours
50 - 60
>80% bases higher than Q30
2 x 150
29 hours
100 - 120
>75% bases higher than Q30
Mid-Output
Read Length (bp)
Total Time
Output (GB)
Quality
2 x 75
15 hours
16 - 19
>80% bases higher than Q30
2 x 150
26 hours
32 - 39
>75% bases higher than Q30

MiSeq

Read Throughput
Reagent Kit V3
Single Reads
22 - 25 million
Paired-end Reads
44 - 50 million
Reagent Kit V2
Single Reads
12 - 15 million
Paired-end Reads
24 - 30 million
Base Throughput
Reagent Kit V3
Read Length (bp)
Total Time
Output (GB)
Quality
2 x 75
24 hours
3.3 - 3.8
>85% bases higher than Q30
2 x 300
65 hours
13.2 - 15
>70% bases higher than Q30
Reagent Kit V2
Read Length (bp)
Total Time
Output (GB)
Quality
2 x 150
24 hours
4.5 - 5.1
>80% bases higher than Q30
2 x 250
39 hours
7.5 - 8.5
>75% bases higher than Q30