Researchers MUST be trained on the following equipment before operating. Sign up for a full training session, or refresher training by clicking below: 

Analysis & QC Instruments

GenCore is equipped with several auxiliary instruments for assessing sample viability, quality, and concentration. 

The Agilent Bioanalyzer 2100 is a micro-fluidics based platform that can analyze the quantity, quality, and characteristics of small RNA, DNA, protein and cell samples.

GenCore has two Agilent Bioanalyzer 2100 systems:

  • 8th Floor, CGSB
  • 8th Floor Brown Building

Quick Reference Guide

The Agilent TapeStation 2200 analyzes RNA and DNA sample quality and fragment length. It offers scalable throughput, is fast and easy to use, and provides researchers with great reproducibility.

Location: 8th Floor, CGSB

Quick Reference Guide

The Agilent TapeStation 4200 is a high throughput automated electrophoresis tool for analyzing nucleic acid sample quality and fragment length. It offers scalable throughput, is fast and easy to use, and provides researchers with great reproducibility.

Location: 8th Floor, CGSB

Quick Reference Guide

The CFX96 qPCR instrument is a powerful and flexible system that combines advanced optical technology with precise temperature control.

Location: 8th Floor, Brown Building

Quick Reference Guide 

The Life Technologies Countess II FL is an automated cell counter with florescence capability. This allows for cell counting, fluorescent protein expression monitoring, and measuring of cell viability.

**EVOS Light Cubes Available: GFP, RFP, & DAPI

Location: 8th Floor, CGSB

Quick Reference Guide

The NanoDrop Spectrophorometer allows the quantification of microvolumes. The Department has a number of these units, including one in the Shared Equipment Core, for general use.

Location: 8th Floor, CGSB

The QuantStudio 3D dPCR (digital PCR) system enables an absolute quantification of samples with extreme precision and sensitivity. The sealed chip technology provides a reliable and robust method for dPCR.

Location: 8th Floor, CGSB

Quick Reference Guide

The Roche LightCycler 480 system consists of a thermal cycler used for rapid real-time quantitative PCR and SNP detection, a computer to control the thermal cycler, and software for the design of probes for hybridization probe analysis and primers for SYBR green analysis. 

Location: 8th Floor, CGSB

Quick Reference Guide

The Typhoon FLA 9000 laser scanner allows for the quantification of proteins, nucleic acids and other bio-molecules, including measurement of radioactive labels, chemifluorescent Western Blots, 2-D DIGE, multiplex fluorescence and colorimetric stains.

Location: 7th Floor, Brown Building

Quick Reference Guide

The YSI 2950D-3 Biochemistry analyzer is equipped with two Biosensor Modules and one ISE Module. It can measure up to 6 chemistries per sample, and is currently set up with the standard configuration to measure glucose, lactate, glutamine, glutamate, ammonium, and potassium (although this can be changed based on user needs). 

Location: 7th Floor, Brown Building

Quick Reference Guide 

Flow Cytometry

There are two high throughput flow cytometers available to sort and analyze various cell types using fluorescence. Technician run cell sorting is also available for researchers.

The BD Accuri is a bench-top flow cytometer that is easy to use and simple to maintain. A sheath-focused core enables event rates of up to 10,000 events per second. For maximum flexibility, the system can accommodate 48- and 96-well plates, deep well plates, and standard 1.5mL tubes.

Location: 8th Floor, CGSB

Quick Reference Guide

SORP Laser Configurations Below:

The FACSAria is a high throughput cell sorter capable of sorting 50,000-70,000 cells per second in up to six channels and into four flow streams. The system contains blue, red, yellow, and violet lasers for excitation of a variety of fluorophores utilizing about 10 channels.

**Please note: Only technician-run appointments are available at this time.

**Be sure to join the GenCore FACSAria User Google Group to be updated on all FACS related issues. CLICK HERE TO JOIN!  

Location: 8th Floor, CGSB

Quick Reference Guide (with laser configurations)

GenCore FACSAria Policies

High-Throughput Instruments

GenCore has a number of instruments used to perform various repetitive tasks in high throughput, including NGS library preparation, normalizations, and cell-based fluorescence assays. 

The 10X Genomics Single Cell Chromium Controller is a benchtop instrument for single cell capture and gene expression profiling. The 10X Chromium can capture hundreds to millions of cells with a simple workflow, and users can go from cell sample to sequencing library in less than one workday.

Location: 8th Floor, CGSB

Quick Reference Guide

The Agilent Bravo Robotics system is a high throughput liquid dispensing robot. It is equipped with a 96 pipette head and a flexible deck that can accommodate Agilent NGS Option B protocols for library preparation, plate stamps, PCR setups, and much more.

**Please contact GenCore if there is a protocol/project you wish to automate.

Location: 8th Floor, CGSB

Quick Reference Guide (along with a list of currently validated protocols!)

The Agilent G2565CA Microarray Scanner System is a highly versatile scanner that can read custom and pre-designed Agilent microarrays. The instrument can read high density spotted arrays (e.g., 8x60k) and has the flexibility of one color or two color scanning.

Location: 8th Floor, CGSB

Quick Reference Guide

The Formulatrix Mantis is a benchtop liquid handler that offers the lowest dead volume of any liquid handler on the market using its patented microfluidic dispensing technology. It is extremely flexible and easy to use, saving time and money.

Location: 8th Floor, CGSB

Quick Reference Guide

The Tecan Spark Microplate Reader is a multimode plate reader that can be used with various plate types ranging from 24-well plates to 384 well plates, allowing scalability for experiments. It is equipped with a high speed monochromator aiding in absorbance, fluorescence, luminescence detection, and more.

Location: 8th Floor, CGSB

Quick Reference Guide

The Sage Blue Pippin an target a wide range of molecular sizes (90bp to 50kb) for use in preparing NGS libraries. 5 samples can be run in parallel with no risk of cross-contamination.

Location: 8th Floor, CGSB

Quick Reference Guide

Protein Detection & Quantification

GenCore has one Mass Spectrometer coupled with a nano-low LC allowing researchers a method for protein identification and quantification.

**Please note: Proteomics services are organized and run by the Vogel Lab.

The Thermofisher Q-Exactive High Field system is coupled with a Nanoflow Easy LC 1200 creating a rapid and robust LC-MS system.

Location: 4th Floor, CGSB

Proteomics Pricing 

Sonicators

There are three different types of sonicators available to researchers for various types of experiments including DNA shearing for NGS, chromatin shearing, tissue homogenization, and more. 

The Covaris ME220 is a high throughput sonicator that allows 8 samples to be analyzed at the bench. It is a compact, reproducible and robust system equipped with AFA-technology.

Location: 8th Floor, CGSB

Quick Reference Guide

The Covaris S-Series Focused Ultra Sonicator is a versatile, high power system that performs at high frequencies for a variety of applications.

Location: 8th Floor, CGSB

Quick Reference Guide

The Diagenode Bioruptor Pico sonicators can uniformly process multiple samples and can be used to shear DNA and chromatin as well as homogenize tissue.

GenCore has two Diagenode Bioruptor Pico Instruments:

  • 8th Floor, CGSB
  • 8th Floor Brown Building

Quick Reference Guide

General Use Equipment

GenCore manages and maintains the following general use equipment that is available to all NYU researchers. These instruments are all located on the 8th Floor of CGSB, unless otherwise specified.